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Comparison of the quantification of caffeine in human plasma by gas chromatography and ELISA BJMBR
Carregaro,A.B.; Woods,W.E.; Tobin,T.; Queiroz-Neto,A..
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according to the criterion of difference, in modulus, of the I50 of the standard curve and of the I50 of the sample. The samples were classified into three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Gas chromatography; ELISA; Caffeine; Plasma; Quantification.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600019
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Effect of amitraz and xylazine on some physiological variables of horses Arq. Bras. Med. Vet. Zootec.
Queiroz-Neto,A.; Carregaro,A.B.; Zamur,G.; Harkins,J.D.; Tobin,T.; Mataqueiro,M.I.; Gonçalves,S.C..
The effect of the intravenous injection of amitraz (0.1mg/kg) to horses on cardiac activity, respiratory rate, intestinal motor activity, rectal temperature, sweating and masticating was evaluated and compared to the effect of intravenous administration of xylazine (1mg/kg, iv) on heart rate, respiratory rate and intestinal activity. Amitraz caused a significant decrease in heart activity, respiratory rate and intestinal movements, but these effects were not as pronounced as those caused by xylazine. Amitraz also caused a significant relaxation of the rectal smooth muscle, and an apparent increase in sweating and in the frequency of horses found masticating hay. Rectal temperature was not influenced by amitraz. The results indicated that amitraz, at the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horse; Amitraz; Xylazine; Cardiac activity; Respiratory activity; Intestinal activity.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100008
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Toxicological evaluation of long-term intravenous administration of amitraz in horses Arq. Bras. Med. Vet. Zootec.
Queiroz-Neto,A.; D'Angelis,F.H.F.; Harkins,J.D.; Tobin,T..
With the aim of determining the possible toxicity of amitraz after its prolonged use in horses, six English Thoroughbred horses received intravenous injections of amitraz (0.05, 0.10 or 0.15 mg/kg) weekly for four months, constituting the experimental group. Eight other animals (control group), via the same route following the same drug administration schedule and period of time, received the vehicle, dimethylformamide. At the end of this period, blood was collected from all the animals, and a comparison was made of the means of the values obtained for the various blood analyses: complete hemogram, alkaline phosphatase, gamma-glutamyltransferase, blood urea nitrogen, lactate dehydrogenase, aspartate aminotransferase, creatine phosphokinase, glucose,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horse; English Thoroughbred; Amitraz; Toxicity; Blood characteristics; Electrolytes.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400003
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